Application and consideration of metabolomics detection in embryo culture medium in the noninvasive screening of embryos / 中华检验医学杂志

In vitro fertilization (IVF) is the most successful and efficient approach to infertility treatment. Choosing the appropriate embryos for transfer significantly decreases the pregnancy failure rate and improves IVF results. Invasive procedure to acquire embryo DNA has a significant limitation. The differences of metabolites, nucleic acids, proteins and other components in embryo medium can help to screen embryos with potential development to improve implantation rate and pregnancy rate, reduce abortion rate and avoid the transfer of aneuploid embryos and the transmission of mutated genes to offspring. The metabolomics screening of high developmental potential embryos with embryo culture medium can assess the developmental potential of embryos comprehensively, objectively and noninvasiveness, which is crucial for improving the pregnancy rate of single embryo transfer.

Diagnostic efficacy comparison between CLLflow score and Moreau score for chronic lymphocytic leukemia / 中华检验医学杂志

Objective:To explore the diagnostic efficacy difference and clinical diagnostic value of chronic lymphocytic leukemia flow (CLLflow) score and Moreau score (MS) in the diagnosis of chronic lymphocytic leukemia (CLL).Methods:According to the latest international and national diagnosis criteria for CLL, 133 patients with B-cell chronic lymphoproliferative diseases and uncertain immunophenotypes (B-CLPD), diagnosed by Zhengzhou Jinyu Comprehensive Haematological Pathology Diagnosis Centre from March 2020 to May 2021, were included in this study. Above patients were divided into the CLL group ( n=83) and non-CLL group ( n=50). The expression of clusters of differentiation (CD)5, CD10, CD20, CD19, κ light chain, λ light chain, FMC7, CD23, CD22, surface immunoglobulin M, CD200 and CD79 were detected by flow cytometry, and CLLflow score and MS score were calculated respectively according to the scoring rules. A fourfold table was used to compare the diagnostic efficacy of the two scoring systems, and the Kappa test and McNemar test were used to compare the consistency and superiority of the systems. Results:The rate of negative and positive CLLflow score were 4.8% (4/83) and 95.2% (79/83) in the CLL group and were 80.0% (40/50) and 20.0% (10/50) in the non-CLL group, and respectively (both P<0.001). The MS score (≤2, =3 and≥4) was 1.2% (1/83), 10.8% (9/83) and 88.0% (73/83) in the CLL group and was 86.0% (43/50), 14.0% (7/50) and 0 in the non-CLL group, there were significant statistical difference between the two groups ( P<0.001). The sensitivity, specificity, positive predictive value and negative predictive value of the CLLflow score were 95.2% (79/83), 80.0% (40/50), 88.8% (79/89) and 90.9% (40/44), respectively and those of MS score were 98.8% (82/83), 86.0% (43/50), 92.1% (82/89) and 97.7% (43/44) respectively. The overall coincidence rate, positive and negative coincidence rate between the CLLflow score and MS score were 91.0% (121/133), 93.3% (83/89) and 86.4% (38/44) respectively. Besides, the McNeamr dominance test presented no significant difference ( P>0.05) and high consistency (Kappa=0.796) between the two scoring systems. With MS≤2 and MS≥4, the sensitivity and the specificity of the MS score were 100% (73/73) and 97.7% (43/44) respectively, and for the CLLflow score, the sensitivity and the specificity were 97.3% (71/73) and 86.4% (38/44) in this MS range. With MS = 3, the sensitivity and specificity of the MS score were 100% (9/9) and 0 (0/7), and CLLflow was 88.9% (8/9) and 57.1% (4/7). Conclusions:The diagnostic efficacy is similar and presents high consistency between the CLLflow score and MS score in CLL diagnosis. For CLL patients with MS = 3, the specificity of MS is relatively low, combined assessment with CLLflow score could improve the diagnosis efficacy for CLL in these patients.

Application and development of molecular diagnostic techniques in human assisted reproduction / 中华检验医学杂志

In the past few years, with the breakthrough development of DNA sequencing technology, preimplantation genetic testing (PGT) has been widely used as an important part of assisted reproductive technology (ART). The progress of next generation sequencing (NGS) technology and the improvement of resolution, as well as the comprehensive application of molecular diagnostic technology makes it possible to perform the extensive and comprehensive chromosome screening and the clinical valuable detection of small gene fragment missing and repetition simultaneously, which is of great significance in terms of improving the pregnancy rate and reducing the abortion rate, multiplets rate and malformation rate. The common PGT molecular diagnostic techniques involves fluorescence in situ hybridization (FISH), single nucleotide polymorphism array(SNP-array), quantitative polymerase chain reaction(qPCR) and NGS. And each of them has its own highlights in clinical application and there are many uncertainties that are difficult to control. Moreover, ethical concerns brought about by technological progress also need to be addressed.

Preparation of different fragments of SARS-CoV-2 N protein and its application in fluorescence chromatography / 生物工程学报

Different fragments of SARS-CoV-2 nucleocapsid (N) protein were expressed and purified, and a fluorescence immunochromatography method for detection of SARS-CoV-2 total antibody was established. The effect of different protein fragments on the performance of the method was evaluated. The N protein sequence was analyzed by bioinformatics technology, expressed in prokaryotic cell and purified by metal ion affinity chromatography column. Different N protein fragments were prepared for comparison. EDC reaction was used to label fluorescence microsphere on the synthesized antigen to construct sandwich fluorescence chromatography antibody detection assay, and the performance was systemically evaluated. Among the 4 prepared N protein fragments, the full-length N protein (N419) was selected as the optimized coating antigen, N412 with 0.5 mol/L NaCl was used as the optimal combination; deleting 91-120 amino acids from the N-terminal of N412 reduced non-specific signal by 87.5%. the linear range of detection was 0.312-80 U/L, the limit of detection was 0.165 U/L, and the accuracy was more than 95%. A fluorescence immunochromatographic detection method for analysis of SARS-CoV-2 total antibody was established by pairing N protein fragments. The detection result achieved 98% concordance with the commercially available Guangzhou Wanfu test strip, which is expected to be used as a supplementary approach for detection of SARS-CoV-2. The assay could also provide experimental reference for improving the performance of COVID-19 antibody detection reagents.

Efficacy analysis of intraperitoneal perfusion combined with intravenous and oral three-way chemotherapy in the treatment of gastric cancer with peritoneal metastasis / 中国综合临床

Objective:To investigate the efficacy of peritoneal perfusion combined with intravenous and oral chemotherapy in the treatment of gastric cancer with peritoneal metastasis and its correlation with prognosis.Methods:The clinical data of 142 patients with peritoneal metastasis of gastric cancer admitted to the Affiliated Hospital of Xuzhou Medical University from March 2012 to June 2019 were retrospectively analyzed.According to different treatment schemes, the patients were divided into observation group (47 cases treated with tegafur capsule orally+ docetaxel+ cisplatin combined with recombinant human tumor necrosis factor intraperitoneal perfusion) and control group (oral administration of tegafur capsule+ oxaliplatin). The clinical efficacy and prognostic factors were compared between the two groups.Results:The short-term efficacy after two courses of chemotherapy: the effective rate was 44.7% (21/47) in the observation group and 8.4% (8/95) in the control group, the difference was statistically significant (χ 2=25.438, P<0.001); the disease control rate was 97.9% (46/47) in the observation group and 60.0% (57/95) in the control group, the difference was statistically significant (χ 2=22.639, P<0.001). Long term efficacy: the progression free survival time of the observation group was higher than that of the control group (6.50 months vs.3.83 months, χ 2=16.399, P<0.001); the total survival time of the observation group was higher than that of the control group (15.60 months vs.10.83 months, χ 2=9.219, P=0.002). Univariate analysis showed that ascites volume, chemotherapy, palliative surgery, liver metastasis, Lauren classification and Her-2 expression were related to prognosis (all P<0.05); there was no significant difference in cumulative survival rate of different gender, age and ECOG score (all P>0.05). Multivariate analysis showed that liver metastasis ( HR=1.996, 95% CI=1.194-3.338, P=0.008 ), Lauren type ( HR=1.979, 95% CI=1.265-3.094, P=0.003 ), Her-2 expression ( HR=3.872, 95% CI=2.202-6.808, P<0.001 ), chemotherapy regimens(whether combined with intraperitoneal chemotherapy) ( HR=1.826, 95% CI=1.046-3.188, P=0.034 ) and abdomina volume ( HR=2.785, 95% CI=1.644-4.718, P<0.001 ) were independent risk factor for the prognosis of patients. Conclusion:Intraperitoneal perfusion combined with intravenous and oral administration has a good clinical effect in the treatment of peritoneal metastasis of gastric cancer, which can effectively improve the clinical symptoms and improve the survival rate of patients.The presence or absence of liver metastasis, Lauren classification, Her-2 expression, chemotherapy method (combined with intraperitoneal perfusion or not) and ascites volume are independent risk factors for the prognosis of peritoneal metastasis of gastric cancer.

Congenital nephrotic syndrome with neonatal polycythemia: a case report / 临床儿科杂志

Objectives To investigate the relationship between congenital nephrotic syndrome (CNS) and neonatal polycythemia, and clinical diagnosis and treatment. Methods The clinical manifestations, diagnosis, and treatment of a case of CNS with neonatal polycythemia were retrospective analyzed, and the related literature were reviewed. Results A male infant had abdominal distention after his birth, followed by feeding intolerance and poor response. On the third day of birth, he was diagnosed of neonatal polycythemia according to the levels of hemoglobin (249 g/L) and hematocrit (0.714 L/L). And after a partial exchange transfusion, the symptoms were improved. On the sixth day of birth, the infant had edema, urinary protein +++, serum albumin at 12.7 g/L and blood cholesterol at 8.84 mmol/L, and was clinical diagnosed of CNS. Oral hormone therapy was ineffective and he died 32 days after birth. Conclusions CNS combined with neonatal erythrocytosis is rare in clinic. The co-existing of the two is more likely to induce thromboembolism and organ dysfunction, and the clinical prognosis is poor.

Correlation study of serum homocysteine with renal function and coagulation indexes in hypertensive patients / 中华检验医学杂志

Objective The purpose of this study was to investigate the correlation of serum homocysteine with renal function and coagulation indexes in hypertensive patients .Methods Through retrospective design , 224 hypertensive patients and 212 healthy subjects who sought medical service in Henan Province Hospital of TCM during 2017 to 2018, were divided into four groups according to hypertension and homocysteine level, that was normotensive normal Hcy group (103 patients), normotensive high Hcy group (109 patients), hypertensive normal Hcy group (115 patients), and hypertensive high Hcy group ( 109 patients ) .Serum homocysteine , serum lipid and renal function indexes were detected by automatic biochemical analyzer .The level of coagulation indexes were detected by automatic coagulation analyzer and platelet was tested by automatic blood cell analyzer .Comparisons of variables between four groups were evaluated by one way ANOVA .The correlation was expressed by the Pearson′s correlation coefficient analysis.Multivariate linear regression analysis was performed to identify variables and influence factor associated with Hcy.Results The concentration of Urea in hypertensive high Hcy group (5.73 ± 1.67)mmol/L was significantly increased compared to normotensive normal Hcy group (4.79 ±1.05)mmol/L (t=3.508, P=0.001).The leve of Urea in hypertensive high Hcy group (5.73 ±1.67) mmol/L was significantly increased compared to normotensive high Hcy group (5.21 ±1.21) mmol/L ( t=1.983, P=0.049) and hypertensive normal Hcy group (4.81 ±1.21)mmol/L (t=3.600, P=0.000).The level of Crea in hypertensive high Hcy group ( 79.52 ±25.92 )μmol/L was significantly increased compared to normotensive normal Hcy group (58.39 ±12.83)μmol/L (t=6.121, P=0.000) and hypertensive normal Hcy group (60.93 ±13.74)μmol/L (t=5.526, P=0.000).The level of UA in hypertensive high Hcy group (389.96 ±96.03)μmol/L were significantly higher than normotensive normal Hcy group (293.65 ± 89.94)μmol/L (t=5.722, P=0.000),normotensive high Hcy group (327.02 ±66.55)μmol/L (t=3.837, P=0.000 ) and hypertensive normal Hcy group ( 291.50 ±73.42 )μmol/L ( t=6.128, P=0.000).The level of BMG,CysC in hypertensive high Hcy group and normotensive high Hcy group were higher than normotensive normal Hcy group and hypertensive normal Hcy group .The level of RBP ,D-Dimer, FDP in hypertensive high Hcy group were significantly higher than that of the other three groups .Serum homocysteine correlated positively with Urea (r=0.276,P=0.000),Crea(r=0.389,P=0.000),UA(r=0.339,P=0.000),BMG(r=0.221,P=0.002),RBP(r=0.396,P=0.000),CysC(r=0.200,P=0.006).Multivariate regression analysis showed that the Hcy level was the influencing factors of Urea , Crea and RBP, and hypertension was the influencing factor of Crea , UA, BMG RBP and CysC. Conclusions Hypertensive patients with hyperhomocysteinemia caused renal injury easily . Serum homocysteine may play an important role in renal injury and further affect the occurrence and development of hypertension by impairing the function of platelet , coagulation and fibrinolysis system .

The significance of procalcitonin and C-reactive protein in the early diagnosis of sepsis after percutaneous nephrolithotomy / 中国医师进修杂志

Objective To investigate the significance of procalcitonin (PCT) and C-reactive protein (CRP) in the early diagnosis of sepsis in patients with percutaneous nephrolithotomy (PCNL). Methods One hundred and ninety-eight patients who had underwent PCNL were divided into sepsis group (148 cases) and control group (50 cases). The PCT and CRP 6 h before and after operation were compared between 2 groups, and the receiver operating characteristic curve was drawn. The area under curve (AUC), optimal diagnostic boundary value, susceptibility and specificity were calculated. Results The PCT and CRP before operation in sepsis group were significantly higher than those in control group:(3.911 ± 2.011) ng/L vs. (1.353 ± 0.311) ng/L and (54.85 ± 8.75) g/L vs. (38.65 ± 10.76) g/L, and there were statistical differences (P 1.65 ng/L, and the susceptibility and specificity were 97.91% and 91.01%; the optimal diagnostic boundary value of CRP was > 43.54 g/L, and the susceptibility and specificity group were 93.51% and 79.21%. Conclusions For patients with sepsis after PCNL, PCT is more significant than CRP for early diagnosis. PCT detection can be used as an important auxiliary examination for clinical diagnosis of sepsis, which could help clinicians to detect and treat the sepsis early and can greatly improve the survival rate of patients.

Curative effect of using spiral pedunculated bladder muscle flap in long ureteral segment defect repairing / 中华泌尿外科杂志

ObjectiveTo investigate the effects of using spiral pedunculated bladder muscle flap ureteroplasty in the treatment of long ureteral segment defects ( ＞ 20 cm).MethodsA retrospective analysis was conducted on the clinical effects of five patients who encountered long ureteral segment defects caused during ureteroscopic lithotripsy.The five patients included three males and two females with an age range from 37 to 59 yrs ( average age 48 ).Four of the cases had defects on the left and one case on the right.Two cases had whole ureteral mucosal avulsion and three cases had whole ureteral ruptur from the pelvis to the bladder junction.Defect lengths measured from 21 to 25 cm( mean length 22.5 cm).All five patients underwent emergency surgery using spiral pedunculated bladder muscle flap ureteroplasty and 7 F double J stent placement in the repaired ureters which was fixed on psoas muscles.The average length of the new ureters using spiral pedunculated bladder muscle flap was 22.5 cm.ResultsAll the operations were successful and the operation time was 1 -2 hrs (average 1.5 hrs).Drainage tubes for four patients were removed three days after operation.IN the remaining case the drainage tube was removed 10 days after surgery due to urine leakage.All wounds healed uneventfully.Serum creatinine and blood urea nitrogen were normal two weeks after surgery.Double-J tubes were removed safely under cystoscope eight weeks after surgery.In following-up,one case was found to have mild hydronephrosis and ipsilateral ureter slight expansion six months after surgery,but renal function was normal.There was no abnomality found in the remaining four patients after 2 -4 years of follow-up.The IVU showed normal morphology and good developments in the ipsilateral ureter.ConclusionsSpiral pedunculated bladder muscle flap ureteroplasty is an ideal treatment method in repairing long ureteral segment defects.

Relationship between traditional Chinese medicine syndrome types and serum HBV DNA level in chronic HBV carriers positive for HBeAg / 南方医科大学学报

<p><b>OBJECTIVE</b>To explore the relationship between the syndrome types in traditional Chinese medicine (TCM) and serum HBV DNA load in chronic HBV carriers positive for HBeAg.</p><p><b>METHODS</b>According to the TCM syndrome types, 185 chronic HBV carriers with HBeAg positivity were classified into single syndrome group (liver Qi depression, kidney Qi deficiency, spleen Qi deficiency, and kidney Yang deficiency), compound syndrome group, and unidentifiable syndrome group; based on the nature of the condition in TCM terms, the patients were classified into excess syndrome group, deficiency syndrome group and comorbidity syndrome group. The serum HBV DNA levels in these cases were analyzed in relation to the TCM syndrome types and disease nature.</p><p><b>RESULTS</b>HBV DNA levels showed no significant difference among the patients with single syndrome, compound syndromes and unidentifiable syndrome (F=0.910, P=0.404), nor among the patients with the 5 different single TCM syndromes (χ²=4.672, P=0.323) or those with different disease nature (F=0.631, P=0.596).</p><p><b>CONCLUSION</b>Serum HBV DNA level can not be considered as the evidence for syndrome differentiation in chronic HBV carriers with positive HBeAg.</p>

Enhanced expression of TLR4 in HepG2 cells after transient and stable HBV transfection / 中国医师杂志

Objective To observe the expression of TLR4 in hepatocellular carcinoma cell line HepG2 after transient and stable HBV genome transfection. Methods Immunofluorescence flow cytometry was used to detect mean fluorescence intensity (MFI) of TLR4 and TLR4 positive cell percentage in hepetocellular carcinoma cell lines HepG2 and HepG2. 2.15. Various doses of HBV DNA plasmid were transfected into HepG2 cells with lipefectamine 2000. Immunofluoroscenee flow cytometry was used to detect MFI of TLR4 and TLR4 positive ceil rate of infected HepG2 ceils. Trypan blue staining was used to examine the sum of living cells. Results MFI of TLR4 and TLR4 positive cell rate of HepG2.2.15 cells were significantly higher than those in HepG2 cells (both P' <0. Ol). MFI of TLR4 and TLR4 positive cell rate of HepG2 cells transfected by various doses of HBV DNA were significantly higher than those in control group (all P' < 0. 01). MFI of TLR4 and TLR4 positive cell rate of infected HepG2 cells were positively correlated with the doses of HBV DNA (both P' <0. 01) and negatively correlated with the sum of living cells (both P' <0. 01). Conclusions Enhanced expression of TLR4 appeared in HepG2 cells with both transient and stable HBV infection, along with reduction of living cells.

In vitro effect of Fructus ligustri lucidi and its monomers on the migration of mouse melanoblasts / 中华皮肤科杂志

Objective To study the effects of Fructus ligustri lucidi and its monomers, tyrosol and oleanotic acid, on the migration of mouse melanoblast cell line (NCCmelb4M5). Methods Cultured NCCmelb4M5 cells were treated with Fructus ligustri lucidi (0.0625, 0.125, 0.25, 0.5, 2 mg/mL), tyrosol (0.02, 0.04, 0.08, 0.16, 0.8 mg/mL) and oleanolic acid (0.0625, 0.125, 0.25, 0.5, 2.5 mg/mL), respectively,for 48 hours followed by the detection of cell proliferation with MTT assay. The working concentration of the three drugs was determined according to the results of MTT assay. Scratch and transwell assays were performed to observe the effect of Fructus ligustri lucidi and its monomers at working concentration on the migration of NCCmelb4M5 cells. Results Based on the results of MTT assay, the working concentration of Fructus ligustri lucidi, tyrosol and oleanolic acid was determined at 0.125 mg/mL, 0.08 mg/mL and 0.0625 mg/mL respectively, and at these concentrations, these drugs exhibited a cytotoxity lower than that of absolute alcohol with no obvious stimulation of cell proliferation. Scratch and transwell assay revealed a promoting effect of both Fructus ligustri lucidi and tyrosol on melanoblast migration (P<0.05), while oleanolic acid had little effect on melanoblast migration. Conclusions The extract of Fructus ligustri lucidi has a significant stimulatory effect on the migration of mouse melanoblasts, and tyrosol may be an active component of Fructus ligustri lucidi associated with confirmative effect on migration of mouse melanoblasts.

Expression of NF-E2 related factor in vitiligo lesions / 中华皮肤科杂志

Objective To investigate the level of NF-E2 related factor 2 (Nrf 2) in vitiligo lesions.Methods Tissue samples were obtained by press suction blisters at lesional and donor sites of 12 patients with vitiligo who were managed with epidermal transplantation. Four lesional samples from the patients were subjected to primary culture and the level of Nrf 2 was detected by AEC immunohistochemistry after 48hours of culture. Western blotting was utilized to further detect the level of cytoplasmic and nuclear Nrf 2 in tissue samples from the other 8 patients with vitiligo. Results Immunohistochemistry revealed that Nrf 2 was predominantly expressed in cytoplasm, rather than nuclei, of keratinocytes in vitiligo lesions compared with the normal skin of patients. The level of nuclear Nrf 2 was significantly lower in lesions than that in normal skin (0.10 ± 0.03 vs 0.26 ± 0.03, P ＜ 0.01) of the patients. In contrast, there was no significant dif- ference in the level of cytoplasmic Nrf2 between lesional and normal skin (0.61 ± 0.03 vs 0.60 ～ 0.02, P ＞0.05) of patients. Conclusion These results reveal an abnormality of nuclear translocation of Nrf 2 in vitili-go lesions.

Thyroid diseases in 585 patients with connective tissue diseases / 中华内分泌代谢杂志

The clinical data of 585 patients with connective tissue diseases (CTD) were retrospectively analyzed. Thyroid diseases were detected in 236 cases (40.3%) , in which the prevalence of hypothyroidism was the highest (47.9%) , followed by euthyroid sick syndrome (33.1%), Hashimoto thyroiditis (11.4%) and hyperthyroidism (5.1%) ect. Determination of thyroid function and autoantibodies to thyroid gland were essential in these patients.

Effects of chemotherapeutic agents on the expression of TLR2 and TLR4 in helmtocellular carcinoma cell lines HeG2 and HepG2.2.15 / 中国医师杂志

Objective To observe the effects of 5-fluorouraeil(5-FU)and eisplatin(DDP)on the expression of Toll-like receptor 2 (TLR2)and Toll-like receptor4(TLR4)in hepatocellular carcinoma cell lines HepG2 and HepG2.2.15.Methods Direct immanotlaorescenee flow cytometry was used to detect mean flubrescence intensity(MFI)of TLR2 and TLR4,and TLR2 and TLR4 positive cell percentage in HepG2 and HepG2.2.15 cells before and after treated with 5-FU.and DDP at various concentrations for 24h,48h and 72h.Results MFI of TLR2 and TLR4.and TLR2 and 11LR4 positive cell percentage in HepG2.2.15 cells were significantly higher than those in HepG2(P＜0.01).After HepG2 and HepG2.2.15 cells were treated with different concentration of 5-FU and DDP,MFI of TLR2 and TLR4,TLR2 and TLR4 positive cell percentage in HepG2 and HepG2.2.15 cells almost had no change.only MFI of TLR2 in HepG2.2.15 cells decreased after cells were treated with 5-FU at the concentrations of 100,200μg/ml and DDP at the concentrations of 20μg/ml for 72h(P＜0.05 for all).Conclusions 5-FU and DDP can not activate TLR2 and TLR4 signal pathway in hepatocellular carcinoma cell lines HepG2 and HepG2.2.15.To find the activated pathway in TLR2 and TLR4 signal pathway,some other methods should be used,and this will be helpful in antieancer therapy.

Effects of matrine on expression of a proliferation-inducing ligand in colorectal cancer cell lines / 中华消化杂志

Objective To study the effect of matrine on the expression of a proliferation-inducing ligand (APRIL) in colorectal cancer cell line (SW480 cell). Methods MTT assay was used to evaluate the inhibitory effect of matrine on SW480 cells. The protein and mRNA levels of APRIL in SW480 cells were determined by immunohistochemistry and real-time fluorescence quantitative PCR (RFQ-PCR). SW480 cells were treated with 0.5,1.0,2.0 mg/ml of matrine for 24 h, 48 h and 72 h. FU and blank were served as drug control and blank control groups, respectively. Results Matrine had obviously inhibitory effect on proliferation of SW480 cells in a time- and dose-dependant manner. The expression of APRIL was strong in SW480 cells. When treated with 50,100,200 ug/ml of FU, the APRIL mRNA levels in SW480 cells raised gradually and reached the highest levels at 72 h after treatment, which were significantly higher than those in blank control group (all P value<0.001). When treated with 0. 5,1.0, 2.0 mg/ml of matrine, the APRIL mRNA levels in SW480 cells increased at 24 h after treatment, which were significantly higher than those in blank control group (all P value<0. 001), and then decreased gradually and almost equal to level of blank control group at 72 h. Conclusion In treatment with FU, the survival cells.may have stronger ability of proliferation due to higher expression of APRIL in SW480 cells. Anti-APRIL therapy might be an important assistant treatment to counter the impact of APRIL. Matrine will not cause persistent increase of APRIL mRNA levels in SW480 cells, so it might be a helpful drug in anti-tumor theraphy.

Effect of Glycyrrhizin on Heptitis B Virus Infection in HepG2.2.15 Cell Line / 中国中医药信息杂志

Objective To investigate the effect of glycyrrhizin(GL) on the expression of hepatitis B virus e antigen(HBeAg),hepatitis B virus surface antigen(HBsAg),HBV DNA levels and cell proliferation in HepG2.2.15 cell line.Methods HBV DNA level was examined by Real-time PCR.HBsAg and HBeAg levels were examined by ELASA,and cell proliferation was examined by MTT before and after stimulated with GL.The GL groups were compared with the blank control group.Results HBsAg levels in the GL groups were inhibited in dose-dependent manner compared with the blank control group(P

Investigation on the Effect of Jieduquyuziyin Methods on Decreasing the Dosage of Glucocorticoid and Protecting the Hypothalamic-pituitary-adrenal Axis in Systemic Lupus Erythematosus / 浙江中医药大学学报

0.05),while with significant difference in the second therapy course(P

Compatible Stability of Levofloxacin with Tinidazole in Glucose Injection / 中国药房

OBJECTIVE:To study the compatible stability of Levofloxacin with Tinidazolc in Glucose Injection.METHO-DS:The contents were determined by UV-spectrophotometry and the appearance and pH value were observed within 8h after mixing of levofloxacin with tinidazolc in glucose injection at room temperature(20℃).RESULTS:The contents,pH value and appearance of the mixed solution showed no significant changes within 8h.CONCLUSION:The mixture of the levofloxacin and tinidazolc in glucose injection can be used within 8h after mixing.

Correlation of acute cerebrovascular diseases with the levels of leukocytes and blood glucose at early stage and recent prognosis / 中国组织工程研究

BACKGROUND: The increase of leukocyte and blood glucose levels is a kind of stress reaction when acute cerebrovascular disease occurs, it often occurs at the early clinical stage of acute cerebrovascular disease.OBJECTIVE: To observe the levels of leukocyte and blood glucose at the early stage of acute cerebrovascular disease, and investigate their correlation with recent prognosis.DESIGN: A prognostic analysis based on diagnosis taken patients as observational subjects.SETTING: Qingtian County People's Hospital.PARTICIPANTS: Totally 235 inpatients with acute cerebrovascular disease aged (63.13±9.55) years were selected from Qingtian County People's Hospital between May 2000 and May 2002, including 90 cases of cerebral hemorrhage (52 males and 38 females) aged (63.13±9.55) years and 145 cases of cerebral infarction (73 males and 72 females) aged (71.22±8.00) years. The disease course ≤ 1 month. According to the number of leukocytes, the patients were divided into normal leukocyte group (n=167) and increased leukocyte group (n=68). According to the level of blood glucose, they were divided into normal blood glucose group (n=146) and hyperglycemia group (n=89). The neurologic deficit score (NDS) of 0-15 points was taken as mild, 16-30 as moderate and 31-45as severe.METHODS: All the patients received cranial CT examination immediately after admission, and fasting venous blood samples were drawn on the next morning to detect the levels of leukocyte and fasting blood glucose. The x2 test was applied.MAIN OUTCOME MEASURES: ① Comparison of age: It was obviously higher in the cerebral infarction group than in the cerebral hemorrhage group [(71.22±8.00), (63.13±9.55) years, t=6.90, P ＜ 0.001]. ② Comparison of the percentage of disease types: In the increased leukocyte group,the percentage of cerebral hemorrhage was obviously higher than that of cerebral infarction (66%, 34%, x2=31.47, P ＜ 0.005). In the hyperglycemia group, the percentage of cerebral hemorrhage was higher than that of cerebral infarction (51%, 49%, x2=9.12, P ＜ 0.005). ③ Comparison of the percentage of severe neurological impairment: It was obviously higher in the increased leukocyte group than in the normal leukocyte group (26%, 6%,x2=21.12, P ＜ 0.005), also markedly higher in the hyperglycemia group than in the normal blood glucose group (27%, 5%, x2=21.70, P ＜ 0.005).④ Comparison of the percentage of death: It was obviously higher in the increased leukocyte group than in the normal leukocyte group (31%, 6%,x2=26.15, P ＜ 0.005), also markedly higher in the hyperglycemia group than in the normal blood glucose group (26%, 5%, x2=20.02, P ＜ 0.005). CONCLUSION: Both the increase of leukocyte and blood glucose can damage the brain tissue, and can be used to judge the disease condition of patients with acute cerebrovascular disease, estimate the prognosis and guide the treatment.